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ANGPTL3 ELISA 试剂盒

Name: ANGPTL3 ELISA 试剂盒
SKU: ABIN4881825
Availability: OutOfStock

This 人 ANGPTL3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 ANGPTL3. This ELISA Kit has been cited in1 publication.

(1 reference)

产品编号 ABIN4881825

发货至: 中国

Quick Overview for ANGPTL3 ELISA 试剂盒 (ABIN4881825)

抗原

See all ANGPTL3 ELISA试剂盒

ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

8.2-2000 pg/mL

应用范围

ELISA

样品类型

Plasma, Cell Culture Supernatant, Serum

Discover our top product ANGPTL3 ELISA Kit

最低检测浓度

8.2 pg/mL

原理

Human ANGPTL3 ELISA Kit for serum, plasma, and cell culture supernatant samples.

Analytical Method

Quantitative

特异性

This ELISA kit shows no cross-reactivity with the following cytokines tested: human beta IG-H3, CA9, Cathepsin B, CD23, CD26 (DPPIV), CHI3L1, Coagulation Factor III, CTLA-4, Dkk-4, EDA-A2, Erythropoietin R, FGF-6, FGF-9, FOLH1 (PSMA), Gas 1, IGFBP-5, IL-38 (IL-1 F10), IL-36 Ra (IL-1 F5), IL-36 alpha (IL-1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-1 R5 (IL-18 R alpha), IL-10 R alpha, IL-17C, IL-18, IL-20, IL-34, IL-5 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, SIGIRR, TGF beta R3,TWEAK.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Angiopoietin-Like 3 (ANGPTL3) ELISA Kit
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Angiopoietin-Like 3 (ANGPTL3) ELISA Kit
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ANGPTL3 适用: 人 AA 17-460 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Angiopoietin-Like 3 (ANGPTL3) ELISA Kit
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Angiopoietin-Like 3 (ANGPTL3) ELISA Kit
ANGPTL3 适用: 小鼠 Colorimetric Competition ELISA 1.0-25 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

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ANGPTL3 适用: Cow Colorimetric Competition ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Angiopoietin-Like 3 (ANGPTL3) ELISA Kit
ANGPTL3 适用: 犬 Colorimetric Competition ELISA 1.0-25 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

应用备注

Recommended Dilution for serum and plasma samples200 fold

样本量

100 μL

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
Bassyouni, Sharaf, Wali, Mansour: "Clinical significance of Angiopoietin-1 in Behcet's disease patients with vascular involvement." in: Heart and vessels, Vol. 31, Issue 6, pp. 918-24, (2017) (PubMed).
抗原 See all ANGPTL3 ELISA试剂盒

ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

别名

ANGPTL3

基因ID

27329

UniProt

Q9Y5C1